Ukumiselwa koMxholo we-Oxalic Acid kwiZiqhamo nakwiMifuno nge-High-Performance Liquid Chromatography (HPLC)
1. Umgaqo wovavanyo kunye noyilo lweendlela
Njenge-asidi eqhelekileyo ye-organic kwiziqhamo nakwimifuno, umxholo we-oxalic acid uchaphazela ngokuthe ngqo incasa kunye nexabiso lesondlo lokutya. Olu vavanyo lusebenzisa i-reversed-phase high-performance liquid chromatography (RP-HPLC). Phantsi kweemeko ze-acidic mobile phase, i-oxalic acid yahlulwe ngokwesiseko kwizinto eziphazamisayo kusetyenziswa ikholamu ye-C18 chromatographic. I-ultraviolet detector esetiweyo kwi-210 nm isetyenziselwa uhlalutyo lobungakanani, ngokusekelwe kwiimpawu zokufunxwa kwe-UV zamaqela e-carboxyl kwiimolekyuli ze-oxalic acid.
2. Igophe eliqhelekileyo kunye noLungiselelo lweSampuli
Indlela yokuxutywa kwe-gradient isetyenziselwa ukulungiselela izisombululo eziqhelekileyo: ngokuchanekileyo linganisa i-25.0 mg ye-oxalic acid dihydrate uze uyixube ukuya kwi-25 mL ngamanzi acocekileyo kakhulu ukuze ufumane isisombululo sesitokhwe se-1 mg/mL. Oku kuxutywa ngokulandelelanayo kuluhlu lwezisombululo eziqhelekileyo kwi-50, 100, 200, 400, kunye ne-800 µg/mL, kunye noxinzelelo ngalunye olufakwe kwi-triplicate.
Ukulungiselela isampuli, kusetyenziswa ukukhupha okuncediswa yi-microwave: linganisa i-5.00 g ye-homogenate yeziqhamo/yemifuno, yongeza i-10 mL yesisombululo se-hydrochloric acid esingu-0.1 mol/L, unyange kwi-60°C nge-microwave imizuzu eli-10, uhluze ngesihluzo se-membrane esingu-0.45 µm, uze uqokelele i-filtrate ukuze ivavanywe.
3. Ukuphucula Iimeko zeChromatographic
Isigaba esihambayo sisistim se-0.01 mol/L potassium dihydrogen phosphate buffer (pH 2.5)–acetonitrile (95:5), esinesantya sokuhamba se-0.8 mL/min kunye nobushushu bekholamu obuyi-35°C. Ukulungisa i-acetonitrile proportions kubonisa ukuba xa isigaba se-organic sidlula i-10%, ixesha lokugcina le-oxalic acid lifutshane liye ngaphantsi kwemizuzu emi-3, kodwa umsila ophezulu uyenzeka. Phantsi kweemeko zokugqibela ezilungisiweyo, ixesha lokugcina le-oxalic acid yimizuzu eyi-4.2, nto leyo efikelela ekuhlukanisweni ngokupheleleyo ne-citric acid ekufutshane kunye ne-malic acid (isisombululo > 1.5).
4. Ukuqinisekiswa kweNdlela
Ukuqinisekiswa koluhlu olunemigca kubonisa ubudlelwane obuhle obunemigca phakathi kwendawo ende kunye noxinzelelo kuluhlu lwe-10–1000 µg/mL (R² = 0.9993). Umda wokufumanisa (LOD), omiselwe yindlela yomlinganiselo wesignali-kwingxolo, yi-0.5 µg/mL. Kwiimvavanyo zokuphinda-phinda, i-RSD yezigqibo ezintandathu eziphindaphindwayo zesampulu yesipinatshi efanayo yi-1.8%. Iimvavanyo zokubuyisela ispike kumanqanaba amathathu (80%, 100%, kunye ne-120%) zivelisa umyinge wokubuyisela we-98.2%, 102.4%, kunye ne-97.8%, ngokwahlukeneyo, ukuhlangabezana neemfuno zohlalutyo lobungakanani.
5. Uhlalutyo lweeSampuli zokwenyani
Iziqhamo nemifuno emithandathu ethengiswayo ivavanyiwe: ispinatshi (356 ± 12 mg/100 g), iseleri (215 ± 9 mg/100 g), itamatato (18 ± 2 mg/100 g), iapile (6 ± 1 mg/100 g), ibhanana (ayibonwanga), kunye nebroccoli (89 ± 5 mg/100 g). Uthelekiso nendlela esemgangathweni kazwelonke (GB 5009.277-2016) lubonisa ukuba ukuphambuka phakathi kwezi ndlela zimbini kungaphantsi kwe-5%, okuqinisekisa ukuthembeka kwale ndlela. Ngokukodwa, indlela ye-HPLC ibonisa uvakalelo oluphezulu kuneendlela zendabuko ze-titration xa kuhlalutywa iisampulu zomxholo ophantsi.
6. Amanqaku aphambili
Lawula ngokungqongqo ixabiso le-pH ngexesha lonyango lwesampulu. Ukuba i-pH yesicatshulwa idlula i-3, ukuhla kwe-calcium oxalate kuya kukhokelela kwiziphumo zovavanyo eziphantsi. Isigaba esijikelezayo kufuneka silungiswe sitsha yonke imihla ukuze kuthintelwe ukuhla kwetyuwa kunye nokuvaleka kwekholamu. Emva kokufakwa rhoqo kwama-20, hlambulula ikholamu ngamanzi e-acetonitrile (30:70) imizuzu engama-30 ukuthintela ukuwohloka kokusebenza kwekholamu. Kwiisampuli ezine-pigment (umz., ikhaphetshu emfusa), kucetyiswa ukuba wongeze inyathelo lokucoca ukukhupha eliqinileyo ngaphambi kokufakwa.
7. Imiyalelo yokusetyenziswa kunye nokwandiswa
Le ndlela inokwandiswa ukuya ekufumaneni i-oxalic acid kwiisampuli zebhayoloji ezifana nomchamo negazi. Ngokulungisa ukwakheka kwesigaba esihambayo (umz., ukongeza i-tetrabutylammonium hydroxide), i-oxalic acid kunye ne-metabolites zayo zinokumiselwa ngaxeshanye. Idityaniswe ne-mass spectrometry detector, indlela echanekileyo yokufumanisa umkhondo inokusekwa. Kwishishini lokucubungula ukutya, le ndlela inokubonelela ngenkxaso yedatha ebalulekileyo ekukhetheni iintlobo ze-oxalic acid ezisezantsi kunye nokwenza ngcono iinkqubo zokupheka.
I-Zibo Anhao Chemical Co., Ltd. – I-Oxalic Acid
I-Zibo Anhao Chemical Co., Ltd. ngumthengisi othembekileyo nothumela ngaphandle i-oxalic acid ekumgangatho ophezulu (umgangatho wezimboni, i-99.6% min) evela eShandong, eTshayina. I-oxalic acid yethu yi-white crystalline solid, eyaziwa ngokuba yi-ethanedioic acid, enefomula yemolekyuli i-C₂H₂O₄. Inombala ococekileyo kakhulu, ukungcola okuphantsi, kunye nomgangatho ozinzileyo kwiibhetshi ezahlukeneyo, ihlangabezana neemfuno zelaphu, isikhumba, isinyithi, ukucubungula umhlaba onqabileyo, ukucoca iikhemikhali, kunye namashishini okwakha.
Sinikezela nge-oxalic acid e-anhydrous kunye ne-dihydrate, efumaneka kwiingxowa ezingama-25kg, iingxowa ezingama-50kg, kunye neengxowa ezinkulu ezingama-1000kg. Ngeenkqubo ezingqongqo ze-QC, iingxelo zovavanyo ezipheleleyo, kunye nezicwangciso zokubonelela eziguquguqukayo, siqinisekisa ukuhanjiswa okuzinzileyo kunye nokusebenza okuzinzileyo kubathengi behlabathi. Iimveliso zethu zithunyelwe ngokubanzi kuMzantsi-mpuma we-Asia, eYurophu, e-Afrika, naseMelika, zifumana ukuqatshelwa komgangatho othembekileyo kunye namaxabiso akhuphisanayo.
Khetha iZibo Anhao Chemical ukuze ufumane i-oxalic acid ethembekileyo kunye nenkonzo yobungcali yeekhemikhali enye.
Khetha iZibo Anhao Chemical ye-oxalic acid ecocekileyo kakhulu enika ukusebenza okuzinzileyo kunye nexabiso kwishishini lakho. Qhagamshelana nathi ukuze ufumane iiodolo ezininzi okanye iinkcukacha zobugcisa: info@anhaochemical.com
